A dilution incubation bioassay for determination of water biostability
Wang Qiu-Hua
,
Tao Tao
,
DOI:
Received November 04, 2013,Revised February 24, 2014, Accepted March 07, 2014, Available online 2014-04-14
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Water biostability is of particular concern to water supply as a major limiting factor for heterotrophic bacterial growth in water distribution systems. This study was focused on bacterial growth dynamics in the series of dilutions of water sample with TOC (total organic carbon) value determined beforehand. The results showed that the specific growth rate of pseudomonas fluorescens P17 varied dramatically and irregularly below the fixed TOC values of the different water samples during the initial periods of incubation in given conditions. According to this relationship, a dilution incubation bioassay was developed for rapid determination of water biostability. In the bioassay, water biostability was higher below the fixed TOC value than over the value, because the ability sustaining more bacterial lives of water sample required higher level of the substrate concentration. The fixed TOC value existed in all experiments with tap water, acetate solution and mixed solution in the similar way. Therefore, it is possible to build a new parameter-TGOC (total growth organic carbon) to assess water biostability. The development of the dilution incubation bioassay with a previous TOC determination of the 0.22 μm filtered water sample can be helpful to provide another alternative for rapid determination of water biostability.
