TY - JOUR ID - 10.1016/S1001-0742(10)60590-6 TI - Simultaneous determination of three classes of antibiotics in the suspended solids of swine wastewater by ultrasonic extraction, solid-phase extraction and liquid chromatography-mass spectrometry AU - Xun Pan AU - Zhimin Qiang AU - Weiwei Ben and Meixue Chen VL - 23 IS - 10 PB - SP - 1729 EP - 1737 PY - JF - Journal of Environmental Sciences JA - J. Environ. Sci. UR - http://www.jesc.ac.cn/jesc_en/ch/reader/view_abstract.aspx?file_no=2011231020&flag=1 KW - antibiotics;swine wastewater;suspended solids;ultrasonic extraction;solid-phase extraction;mass spectrometry KW - antibiotics;swine wastewater;suspended solids;ultrasonic extraction;solid-phase extraction;mass spectrometry AB - This work describes a systematic approach to the development of a method for simultaneous determination of three classes of veterinary antibiotics in the suspended solids (SS) of swine wastewater, including five sulfonamides, three tetracyclines and one macrolide (tiamulin). The entire procedures for sample pretreatment, ultrasonic extraction (USE), solid-phase extraction (SPE), and liquid chromatography-mass spectrometry (LC-MS) quantification were examined and optimized. The recovery efficiencies were found to be 76%-104% for sulfonamides, 81%-112% for tetracyclines, and 51%-64% for tiamulin at three spiking levels. The intra-day and inter-day precisions, as expressed by the relative standard deviation (RSD), were below 17%. The method detection limits (MDLs) were between 0.14 and 7.14 μg/kg, depending on a specific antibiotic studied. The developed method was applied to field samples collected from three concentrated swine feeding plants located in Beijing, Shanghai and Shandong province of China. All the investigated antibiotics were detected in both SS and liquid phase of swine wastewater, with partition coefficients (logKd) ranging from 0.49 to 2.30. This study demonstrates that the SS can not be ignored when determining the concentrations of antibiotics in swine wastewater. ER -