TY - JOUR
ID - 10.1016/S1001-0742(11)60861-9
TI - Spatial distribution of archaeal and bacterial ammonia oxidizers in the littoral buffer zone of a nitrogen-rich lake
AU - Yu Wang
AU - Guibing Zhu
AU - Lei Ye
AU - Xiaojuan Feng
AU - Huub J. M. Op den Camp and Chengqing Yin
VL - 24
IS - 5
PB -
SP - 790
EP - 799
PY -
JF - Journal of Environmental Sciences
JA - J. Environ. Sci.
UR - http://www.jesc.ac.cn/jesc_en/ch/reader/view_abstract.aspx?file_no=2012240502&flag=1
KW - littoral zone;ammonia oxidation;archaea;bacteria;heterogeneity;abundance
KW - littoral zone;ammonia oxidation;archaea;bacteria;heterogeneity;abundance
AB - The spatial distribution and diversity of archaeal and bacterial ammonia oxidizers (AOA and AOB) were evaluated targeting amoA genes in the gradient of a littoral buffer zone which has been identified as a hot spot for N cycling. Here we found high spatial heterogeneity in the nitrification rate and abundance of ammonia oxidizers in the five sampling sites. The bacterial amoA gene was numerically dominant in most of the surface soil but decreased dramatically in deep layers. Higher nitrification potentials were detected in two sites near the land/water interface at 4.4-6.1 μg NO2--N/(g dry weight soil·hr), while only 1.0-1.7 μg NO2--N/(g dry weight soil·hr) was measured at other sites. The potential nitrification rates were proportional to the amoA gene abundance for AOB, but with no significant correlation with AOA. The NH4 + concentration was the most determinative parameter for the abundance of AOB and potential nitrification rates in this study. Higher richness in the surface layer was found in the analysis of biodiversity. Phylogenetic analysis revealed that most of the bacterial amoA sequences in surface soil were affiliated with the genus of Nitrosopira while the archaeal sequences were almost equally affiliated with Candidatus ‘Nitrososphaera gargensis’ and Candidatus ‘Nitrosocaldus yellowstonii’. The spatial distribution of AOA and AOB indicated that bacteria may play a more important role in nitrification in the littoral buffer zone of a N-rich lake.
ER -