TY - JOUR ID - 10.1016/S1001-0742(11)61064-4 TI - Degradation of polycyclic aromatic hydrocarbons by Pseudomonas sp. JM2 isolated from active sewage sludge of chemical plant AU - Jing Ma AU - Li Xu and Lingyun Jia VL - 24 IS - 12 PB - SP - 2141 EP - 2148 PY - JF - Journal of Environmental Sciences JA - J. Environ. Sci. UR - http://www.jesc.ac.cn/jesc_en/ch/reader/view_abstract.aspx?file_no=2012241214&flag=1 KW - polycyclic aromatic hydrocarbons;Pseudomonas sp.;biodegradation;formate;salicylate KW - polycyclic aromatic hydrocarbons;Pseudomonas sp.;biodegradation;formate;salicylate AB - It is important to screen strains that can decompose polycyclic aromatic hydrocarbons (PAHs) completely and rapidly with good adaptability for bioremediation in a local area. A bacterial strain JM2, which uses phenanthrene as its sole carbon source, was isolated from the active sewage sludge from a chemical plant in Jilin, China and identified as Pseudomonas based on 16S rDNA gene sequence analysis. Although the optimal growth conditions were determined to be pH 6.0 and 37℃, JM2 showed a broad pH and temperature profile. At pH 4.5 and 9.3, JM2 could degrade more than 40% of fluorene and phenanthrene (50 mg/L each) within 4 days. In addition, when the temperature was as low as 4℃, JM2 could degrade up to 24% fluorene and 12% phenanthrene. This showed the potential for JM2 to be applied in bioremediation over winter or in cold regions. Moreover, a nutrient augmentation study showed that adding formate into media could promote PAH degradation, while the supplement of salicylate had an inhibitive effect. Furthermore, in a metabolic pathway study, salicylate, phthalic acid, and 9-fluorenone were detected during the degradation of fluorene or phenanthrene. In conclusion, Pseudomonas sp. JM2 is a high performance strain in the degradation of fluorene and phenanthrene under extreme pH and temperature conditions. It might be useful in the bioremediation of PAHs. ER -