Biodegradation of azo dyes by genetically engineered azoreductase

WANG Jing , YAN Bin , ZHOU Ji-ti , BAO Yong-ming , LU Hong , YUAN Xiao-dong


Received ,Revised , Accepted , Available online

Volume 17,2005,Pages 545-550

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A azoreductase gene with 537 bp was obtained by PGR amplification from Rhodobacter sphaeroides AS1.1737. The enzyme,with a molecular weight of 18.7 kD, was efficiently expressed in Escherichia coli and its biodegradation characteristics for azo dyes were investigated. Furthermore, the reaction kinetics and mechanism of azo dyes catalyzed by the genetically engineered azoreductase were studied in detail. The presence of a hydrazo-intermediate was identified, which provided a convincing evidence for the assumption that azo dyes were degraded via an incomplete reduction stage.

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