Aquatic environment

Potential of plant polyphenol oxidases in the decolorization and removal of textile and non-textile dyes


AMJAD ALI Khan , QAYYUM Husain

DOI:

Received April 21, 2006,Revised July 16, 2006, Accepted , Available online

Volume 19,2007,Pages 396-402

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In this study an e ort has been made to use plant polyphenol oxidases; potato (Solanum tuberosum) and brinjal (Solanum melongena), for the treatment of various important dyes used in textile and other industries. The ammonium sulphate fractionated enzyme preparations were used to treat a number of dyes under various experimental conditions. Majority of the treated dyes were maximally decolorized at pH 3.0. Some of the dyes were quickly decolorized whereas others were marginally decolorized. The initial first hour was su cient for the maximum decolorization of dyes. The rate of decolorization was quite slow on long treatment of dyes. Enhancement in the dye decolorization was noticed on increasing the concentration of enzymes. The complex mixtures of dyes were treated with both preparations of polyphenol oxidases in the bu ers of varying pH values. Potato polyphenol oxidase was significantly more e ective in decolorizing the dyes to higher extent as compared to the enzyme obtained from brinjal polyphenol oxidase. Decolorization of dyes and their mixtures, followed by the formation of an insoluble precipitate, which could be easily removed simply by centrifugation.

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