Environmental biology

A novel acidophile community populating waste ore deposits at an acid mine drainage site

HAO Chun-bo , ZHANG Hong-xun , BAI Zhi-hui , HU Qing , ZHANG Bao-guo


Received March 24, 2006,Revised May 17, 2006, Accepted , Available online

Volume 19,2007,Pages 444-450

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Waste ore samples (pH 3.0) were collected at an acid mine drainage (AMD) site in Anhui, China. The present acidophilic microbial community in the waste ore was studied with 16S rRNA gene clone library and denaturing gradient gel electrophoresis (DGGE). Eighteen di erent clones were identified and a liated with Actinobacteria, low G + C Gram-positives, Thermomicrobia, Acidobacteria, Proteobacteria, candidate division TM7, and Planctomycetes. Phylogenetic analysis of 16S rRNA gene sequences revealed a diversity of acidophiles in the samples that were mostly novel. It is unexpected that the moderately thermophilic acidophiles were abundant in the acidic ecosystem and may play a great role in the generation of AMD. The result of DGGE was consistent with that of clone library analysis. These findings help in the better understanding of the generation mechanism of AMD and in developing a more e cient method to control AMD.

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