Environmental biology

Isolates identification and characteristics of microorganisms in biotricklingfilter and biofilter system treating H2S and NH3

YU Guang-hui , XU Xiao-jun , HE Pin-jing


Received September 19, 2006,Revised November 17, 2006, Accepted , Available online

Volume 19,2007,Pages 859-863

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A combination system of biotrickling filter (BTF) and biofilter (BF), adopting surfactant-modified clinoptilolite and surfactantmodified wood chip as the media respectively, was applied to treat H2S and NH3 simultaneously. The identification and sole carbon sources utilization patterns of isolates in the combination system were studied by Biolog system. The isolates were identified as Bacillus sphaericus, Geobacillus themoglucosidasius (55°C) and Micrococcus luteus (ATCC 9341) in BTF, and Aspergillus sydowii (Bainier & Sartory) Thom & Church in BF. Among 95 substrate classes supplied by Biolog system, the carboxylic acids and methyl esters had the highest utilization extent for the four species, followed by the amino acids and peptides. The descending sequence of carbon sources utilization capability of isolates was A. sydowii (52.6%), M. luteus (39.5%), B. sphaericus (21.6%), and G. thermoglucosidasius (17.7%).

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