Evaluation of media for simultaneous enumeration of total coliformand Escherichia coli in drinking water suppliesby membrane filtration techniques
WANG Dunling , FIESSEL Wanda
DOI:
Received June 16, 2007,Revised August 23, 2007, Accepted , Available online
Volume 20,2008,Pages 273-277
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This study evaluated three di erent dehydrated media for simultaneous detection and enumeration of total coliform (TC) and
Escherichia coli in drinking water samples with a standard membrane filtration procedure. The experiment indicated that the di erential
coliform agar (DCA) medium was the most e ective among the tested media in enumerating TC and E. coli, without the need for
extensive accompanying confirmation tests. The results for DCA medium were highly reproducible for both TC and E. coli with
standard deviation of 6.0 and 6.1, respectively. A high agreement (82%) was found between DCA and m-Endo media on 152 drinking
water samples in terms of TC positive. The DCA medium also reduced concealment of background bacteria.
