A proteomic analysis of rice seedlings respondingto 1,2,4-trichlorobenzene stress
GE Cailin , WAN Dingzhen , WANG Zegang , DING Yan , WANG Yulong , SHANG Qi , MA Fai , LUO Shishi
DOI:
Received May 19, 2007,Revised July 18, 2007, Accepted , Available online
Volume 20,2008,Pages 309-319
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The proteomic analysis of rice (Oryza sativa L.) roots and leaves responding to1,2,4-trichlorobenzene (TCB) stress was carried out
by two dimensional gel electrophoresis, mass spectrometric (MS), and protein database analysis. The results showed that 5 mg/L TCB
stress had a significant e ect on global proteome in rice roots and leaves. The analysis of the category and function of TCB stress
inducible proteins showed that di erent kinds of responses were produced in rice roots and leaves, when rice seedlings were exposed to
5 mg/L TCB stress. Most responses are essential for rice defending the damage of TCB stress. These responses include detoxication of
toxic substances, expression of pathogenesis-related proteins, synthesis of cell wall substances and secondary compounds, regulation of
protein and amino acid metabolism, activation of methionine salvage pathway, and also include osmotic regulation and phytohormone
metabolism. Comparing the TCB stress inducible proteins between the two cultivars, the -glucosidase and pathogenesis-related protein
family 10 proteins were particularly induced by TCB stress in the roots of rice cultivar (Oryza sativa L.) Aizaizhan, and the glutathione
S-transferase and aci-reductone dioxygenase 4 were induced in the roots of rice cultivar Shanyou 63. This may be one of the important
mechanisms for Shanyou 63 having higher tolerance to TCB stress than Aizaizhan.
