Eubacteria and Archaea community of simultaneous methanogenesis anddenitrification granular sludge
SUN Yujiao , ZUO Jiane , CHEN Lili , WANG Yong
DOI:
Received July 19, 2007,Revised October 26, 2007, Accepted , Available online
Volume 20,2008,Pages 626-631
- Summary
- References
- Related Articles
Based on the successful performance of a lab-scale upflow anaerobic sludge blanket (UASB) reactor with the capacity of
simultaneous methanogenesis and denitrification (SMD), the specific phylogenetic groups and community structure of microbes in
the SMD granule in the UASB reactor were investigated by the construction of the Eubacteria and Archaea 16S rDNA clone libraries,
fragment length polymorphism, and sequence blast. Real time quantitative-polymerase chain reaction (RTQ-PCR) technique was used
to quantify the contents of Eubacteria and Archaea in the SMD granule. The contents of some special predominant methanogens were
also investigated. The results indicated that the Methanosaeta and Methanobacteria were the predominant methanogens in all Archaea
in the SMD granule, with contents of 71.59% and 22.73% in all 88 random Archaea clones, respectively. The diversity of Eubacteria
was much more complex than that of Archaea. The low GC positive gram bacteria and "-Protebacteria were the main predominant
Eubacteria species in SMD granule, their contents were 49.62% and 12.03% in all 133 random Eubacteria clones respectively. The
results of RTQ-PCR indicated that the content of Archaea was less than Eubacteria, the Archaea content in total microorganisms in
SMD granule was about 27.6%.
