Dynamic changes of microbial community diversity in a photohydrogenproducing reactor monitored by PCR-DGGE
YING Yanling , LV Zhenmei , MIN Hang , CHENG Jun
DOI:
Received November 12, 2007,Revised January 18, 2008, Accepted , Available online
Volume 20,2008,Pages 1118-1125
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A PCR-DGGE (denaturing gradient gel electrophoresis of polymerase chain reaction) protocol was used for monitoring the dynamic
changes in the microbial population during photohydrogen production. Total DNA was extracted directly from the mixed bacterial
community in the reactor and subjected to PCR with V3-16S rDNA and pufM gene primers, and the amplifications were then
analyzed by DGGE. The DGGE patterns demonstrated the dynamics of community structure and the shift of microbial diversity,
which corresponded to di erent running periods of the reactor. The optimal hydrogen producing community formed on day 10. Using
DGGE analysis with the pufM gene fragments was superior to V3-16S rDNA region genes for detecting the dynamic variations of the
photosynthetic bacteria population during hydrogen production. The comparative sequence analysis of excised DGGE bands showed
the relationship between specific population structures and system performance. Rhodopseudomonas palustris was presumed as one of
the dominant community members for hydrogen production in the reactor. The PCR-DGGE protocol was proven to be a good tool for
monitoring the photohydrogen production in real time and o ered the available information to improve the photohydrogen producing
system.
