Isolation and characteristics of a novel biphenyl-degrading bacterial strain,Dyella ginsengisoli LA-4
LI Ang , QU Yuanyuan , ZHOU Jiti , GOU Min
DOI:
Received May 15, 2008,Revised May 15, 2008, Accepted , Available online
Volume 21,2009,Pages 211-217
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A novel biphenyl-degrading bacterial strain LA-4 was isolated from activated sludge. It was identified as Dyella ginsengisoli
according to phylogenetic similarity of 16S rRNA gene sequence. This isolate could utilize biphenyl as sole source of carbon and
energy, which degraded over 95 mg/L biphenyl within 36 h. The major metabolites formed from biphenyl, such as 2-hydroxy-6-oxo-6-
phenylhexa-2,4-dienoic acid (HOPDA) and benzoic acid, were identified by LC-MS. The crude cell extract of strain LA-4 exhibited the
activity of 2,3-dihydroxybiphenyl 1,2-dioxygenase (2,3-DHBD) and the kinetic parameters Km was 26.48 mol/L and Vmax was 8.12
U/mg protein. A conserved region of the biphenyl dioxygenase gene bphA1 of strain LA-4 was amplified by PCR and confirmed by
DNA sequencing.
