Alteration of microbial properties and community structure in soils exposed tonapropamide
GUO Hua , CHEN Guofeng , LV Zhaoping , ZHAO Hua , YANG Hong
DOI:
Received May 02, 2008,Revised September 02, 2008, Accepted , Available online
Volume 21,2009,Pages 494-502
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The effect of pesticide napropamide (N,N-diethyl-2-(1-naphthalenyloxy) propanamide) on soil microorganisms for long-term (56 d)
was assessed by monitoring changes in soil microbial biological responses. Soils were treated with napropamide at 0, 2, 10, 20, 40, and
80 mg/kg soil and sampled at intervals of 1, 3, 7, 14, 28, 42, and 56 d. The average microbial biomass C declined in napropamide-treated
soils as compared to control. The same trend was observed on microbial biomass N after napropamide application.We also determined
the basal soil respiration (BSR) and observed a high level in soils treated with napropamide during the first 7 d of experiment. But
with the passage of incubation time, BSR with napropamide decreased relatively to control. Application of napropamide at 2–80
mg/kg soil had inhibitory e ects on the activity of urease and invertase. Activity of catalase was enhanced during the initial 7 d
of napropamide application, but soon recovered to the basal level. The depressed enzyme activities might be due to the toxicity of
napropamide to the soil microbial populations. To further understand the e ect of napropamide on microbial communities, a PCRDGGE-
based experiment and cluster analysis of 16S rDNA community profiles were performed. Our analysis revealed an apparent
di erence in bacterial-community composition between the napropamide treatments and control. Addition of napropamide apparently
increased the number of bands during the 7–14 d of incubation. These results imply that napropamide-induced toxicity was responsible
for the disturbance of the microbial populations in soil.
