Enzymatic treatment of estrogens and estrogen glucuronide
Takaaki Tanaka , Toshiyuki Tamura , Yuuichi Ishizaki , Akito Kawasaki , Tomokazu Kawase , Masahiro Teraguchi , Masayuki Taniguchi
DOI:
Received ,Revised , Accepted , Available online
Volume 21,2009,Pages 731-735
- Summary
- References
- Related Articles
Natural and synthetic estrogens from sewage treatment systems are suspected to influence the reproductive health of the animals in the
rivers. In this article, we investigated the enzymatic treatment of three estrogens (estrone, 17 -estradiol, and 17 -ethynyletstradiol) by
a fungal laccase which oxidize phenolic compounds with dissolved oxygen. The elimination of the estrogenic activities by enzymatic
oxidation was demonstrated by medaka vitellogenin assay. In addition, we developed an enzymatic treatment system comprised of
-D-glucuronidase and the laccase for 17 -estradiol 3-( -D-glucuronide) degradation. The two enzymes eliminated 17 -estradiol 3-( -
D-glucuronide) and the intermediate, 17 -estradiol, e ciently.
