Variation of peroxidase isoenzyme and biofilm of Phanerochaete chrysosporiumin continuous membrane bioreactor for ReactiveBrilliant Red X3-B treatment
GAO Shang , CHEN Cheng , TAO Fang , HUANG Minsheng , MA Lihua , WANG Zhonghua , WU Linhui
DOI:
Received July 20, 2008,Revised November 14, 2008, Accepted , Available online
Volume 21,2009,Pages 940-947
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The influence of a Reactive Brilliant Red X-3B (RBR X-3B) dye on the peroxidase isoenzyme of Phanerochaete chrysosporium was
determined, and the biofilm structure in a white rot fungal continuous membrane bioreactor (MBR) was also investigated by scanning
electron microscope (SEM). The variation of peroxidase isoenzyme and the decolorization rate in the continuous MBR were evaluated.
The results showed that the 100 mg/L RBR X-3B could stimulate the production of the peroxidase isoenzyme in the shaking-flask
culture. In addition, two new peroxidase isoenzyme bands with relative mobility (Rf) value of 0.27 and 0.28 appeared, but the activity
was lower than the blank control of 11 d. In the continuous MBR, the system worked stably during the first 60 d, the main peroxidase
isoenzyme bands existed and three new bands with Rf value of 0.10, 0.27, and 0.28 appeared. Meanwhile, the biofilm grew well and
the average decolorization rate could reach 90.6%. But the bands of peroxidase isoenzyme decreased rapidly at day 65, only two bands
with Rf value 0.24 and 0.26 existed, and the decolorization rate decreased to 78.3%. Therefore, 5 bottles of P. chrysosporium mycelial
pellet were added into the MBR, and then the activity of the peroxidase isoenzyme and the decolorization rate had a slight recovery.
Finally, the decolorization rate finally decreased to 75.2%. These results contribute to a comprehensive understanding of the variation
of peroxidase isoenzyme and biofilm in continuous MBR by white rot fungi.
