Aquatic environment

Kinetics and mechanisms of p-nitrophenol biodegradation byPseudomonas aeruginosa HS-D38

ZHENG Yongliang , LIU Deli , LIU Shiwang , XU Shangying , YUAN Yongze , XIONG Li


Received November 20, 2008,Revised April 03, 2009, Accepted , Available online

Volume 21,2009,Pages 1194-1199

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The kinetics and mechanisms of p-nitrophenol (PNP) biodegradation by Pseudomonas aeruginosa HS-D38 were investigated. PNP could be used by HS-D38 strain as the sole carbon, nitrogen and energy sources, and PNP was mineralized at the maximum concentration of 500 mg/L within 24 h in an mineral salt medium (MSM). The analytical results indicated that the biodegradation of PNP fit the first order kinetics model. The rate constant kPNP is 2.039 10??2/h in MSM medium, KPNP+N is 3.603 10??2/h with the addition of ammonium chloride and KPNP+C is 9.74 10??3 /h with additional glucose. The addition of ammonium chloride increased the degradation of PNP. On the contrary, the addition of glucose inhibited and delayed the biodegradation of PNP. Chemical analysis results by thin-layer chromatography (TLC), UV-Vis spectroscopy and gas chromatography (GC) techniques suggested that PNP was converted to hydroquinone (HQ) and further degraded via 1,2,4-benzenetriol (1,2,4-BT) pathway.

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