Isolation and characterization of a profenofos degrading bacterium
Saadatullah Malghani , Nivedita Chatterjee , Xueyu Hu , Lou Zejiao
DOI:
Received December 24, 2008,Revised April 23, 2009, Accepted , Available online
Volume 21,2009,Pages 1591-1597
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Profenofos, a well known organophosphate pesticide, has been in agricultural use over the last two decades for controlling
Lepidopteron pests of cotton and tobacco crops. In this study, a bacterial strain, OW, was isolated from a long term profenofos exposed
soil by an enrichment technique, and its ability to degrade profenofos was determined using gas chromatography. The isolated strain
OW was identified as Pseudomonas aeruginosa according to its physiological and biochemical properties, and the analysis of its 16S
rRNA gene sequence. The strain grew well at pH 5.5–7.2 with a broad temperature profile. Bioremediation of profenofos-contaminated
soil was examined using soil treated with 200 g/g profenofos, which resulted in a higher degradation rate than control soils without
inoculation. In a mineral salt medium (FTW), removal in the level of profenofos of 86.81% was obtained within 48 h of incubation.
The intermediates of profenofos metabolism indicated that the degradation occurred through a hydrolysis mechanism, and one of the
metabolites was found to be 4 bromo-2-cholorophenol (BCP) which in turn was also mineralized by the strain. The results of this study
highlighted the potentiality of P. aeruginosa as a biodegrader which could be used for the bioremediation of profenofos contaminated
soil.
