Community analysis of ammonia-oxidizing bacteria in activated sludge of eightwastewater treatment systems
Xiaohui Wang , Xianghua Wen , Craig Criddle , George Wells , Jie Zhang , Yin Zhao
DOI:
Received June 20, 2009,Revised September 22, 2009, Accepted , Available online
Volume 22,2010,Pages 627-634
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We investigated the communities of ammonia-oxidizing bacteria (AOB) in activated sludge collected from eight wastewater treatment
systems using polymerase chain reaction (PCR) followed by terminal restriction fragment length polymorphism (T-RFLP), cloning,
and sequencing of the -subunit of the ammonia monooxygenase gene (amoA). The T-RFLP fingerprint analyses showed that di erent
wastewater treatment systems harbored distinct AOB communities. However, there was no remarkable di erence among the AOB TRFLP
profiles from di erent parts of the same system. The T-RFLP fingerprints showed that a full-scale wastewater treatment plant
(WWTP) contained a larger number of dominant AOB species than a pilot-scale reactor. The source of influent a ected the AOB
community, and the WWTPs treating domestic wastewater contained a higher AOB diversity than those receiving mixed domestic and
industrial wastewater. However, the AOB community structure was little a ected by the treatment process in this study. Phylogenetic
analysis of the cloned amoA genes clearly indicated that all the dominant AOB in the systems was closely related to Nitrosomonas spp.
not to Nitrosospira spp. Members of the Nitrosomonas oligotropha and Nitrosomonas communis clusters were found in all samples,
while members of Nitrosomonas europaea cluster occurred in some systems.
