Environmental biology

Production of a newly isolated Paenibacillus polymyxa biocontrol agent usingmonosodium glutamate wastewater and potato wastewater


Likun Gu , Zhihui Bai , Bo Jin , Jianyun Zhang , Wenying Li , Guoqiang Zhuang , Hongxun Zhang

DOI:

Received October 12, 2009,Revised December 16, 2009, Accepted , Available online

Volume 22,2010,Pages 1407-1412

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A phyllosphere bacterial strain EBL-06 was isolated from wheat leaves. The morphology, cultural characteristics, phospholipid fatty acids, physiological and antagonistic fungus activities of this strain were investigated. A phylogenetic tree was constructed by comparing with the published 16S rDNA sequences of the relevant bacteria. The results showed that the isolate EBL-06 was a strain of Paenibacillus polymyxa; this strain performed a high level of antagonistic fungus activity toward a broad spectrum of phytopathogens, such as Botrytis cinerea, Cladosporium cucumerinum, Fusarium spp. The isolate EBL-06 can grow well using monosodium glutamate wastewater (MGW) and potato wastewater (PW) as culture medium. The maximum yield of 6.5 109 CFU/mL of the isolate EBL-06 anti-fungus biocontrol agent was reached in 15 hr cultivation at 28°C, pH 6.0–7.5 using the mixture of MGW and PW (1:9).

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