Environmental analytical methods

Construction of WCB-11: A novel phiYFP arsenic-resistantwhole-cell biosensor

Qing Hu , Li Li , Yanjuan Wang , Wenjuan Zhao , Hongyan Qi , Guoqiang Zhuang


Received October 13, 2009,Revised January 10, 2010, Accepted , Available online

Volume 22,2010,Pages 1469-1474

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The prediction and assessment of environmental pollution by arsenic are important preconditions of advocating environmental protection and human health risk assessment. A yellow fluorescent protein-based whole-cell biosensor for the detection of arsenite and arsenate was constructed and tested. An arsenic-resistant promoter and the regulatory gene arsR were obtained by PCR from the genome of Escherichia coli DH5 , and phiYFP was introduced into E. coli DH5 as a reporter gene to construct an arsenic-resistant whole-cell biosensor (WCB-11) in which phiYFP was expressed well for the first time. Experimental results demonstrated that the biosensor has a good response to arsenic and the expression of phiYFP. When strain WCB-11 was exposed to As3+ and As5+, the expression of yellow fluorescence was time-dependent and dose-dependent. This engineered construct is expected to become established as an inexpensive and convenient method for the detection of arsenic in the field.

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