Impacts of algal blooms removal by chitosan-modified soils on zooplanktoncommunity in Taihu Lake, China
Jiajia Ni , Yuhe Yu , Weisong Feng , Qingyun Yan , Gang Pan , Bo Yang , Xiang Zhang , Xuemei Li
DOI:
Received November 16, 2009,Revised March 03, 2010, Accepted , Available online
Volume 22,2010,Pages 1500-1507
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It is important to assess the e ect on zooplankton when perform the environmental protection or restoration technology, especially
removing algal blooms, because algae were the major primary producer in algal lakes. The influence on zooplankton community after
half a year of algal blooms removed by chitosan-modified soils in Taihu Lake was assessed and the rationality of carrying out the
process semiannually was evaluated in the present study. Morphological composition and genetic diversity of zooplankton community
were investigated by microscope checkup and polymerase chain reaction-denatured gradient gel electrophoresis (PCR-DGGE). A total
of 44 zooplankton taxa (23 protozoa, 17 rotifers, 3 copepoda and 1 cladocera) were detected by microscope checkup, and a total of
91 bands (28 bands amplified by primers F1427-GC and R1616, 63 bands amplified by primers Fung-GC and NS1) were detected by
PCR-DGGE. The results of cluster analysis or detrended correspondence analysis indicated that there was no considerable di erence
in morphological composition of zooplankton and DGGE profiles between experimental and control sites, and DGGE profiles could
represent the biologic diversity. The study showed that zooplankton community could recover original condition after half year of algal
blooms removed by chitosan-modified soils and it was acceptable to apply this process semiannually. In addition, the results revealed
that PCR-DGGE could be applied to investigate the impacts of the environmental protection or restoration engineering on zooplankton
community diversity.
