Immobilization of an enzyme from a Fusarium fungus WZ-Ifor chlorpyrifos degradation
Hui Xie , Lusheng Zhu , Tingting Ma , Jun Wang , Jinhua Wang , Jun Su , Bo Shao
DOI:
Received December 03, 2009,Revised April 08, 2010, Accepted , Available online
Volume 22,2010,Pages 1930-1935
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The free enzyme extracted from WZ-I, which was identified as Fusarium LK. ex Fx, could e ectively degrade chlorpyrifos, an
organophosphate insecticide. The methods of immobilizing this free enzyme and determined its degradation-related characteristics were
investigated. The properties of the immobilized enzyme were compared with those of the free enzyme. The optimal immobilization of
the enzyme was achieved in a solution of 30 g/L sodium alginate at 4°C for 4–12 hr. The immobilized enzyme showed the maximal
activity at pH 8.0, 45°C. The maximum initial rate and the substrate concentration of the immobilized enzyme were less than that
of the free enzyme. The immobilized enzyme, therefore, had a higher capacity to withstand a broader range of temperatures and pH
conditions than the free enzyme. With varying pH and temperatures, the immobilized enzyme was more active than the free enzyme in
the degradation reaction. In addition, the immobilized enzyme exhibited only a slight loss in its initial activity, even after three repeated
uses. The results showed that the immobilized enzyme was more resistant to di erent environmental conditions, suggesting that it was
viable for future practical use.
