First report of microcystin production in Microcystis smithii Komárek andAnagnostidis (Cyanobacteria) from a water bloom in Eastern China
Yang Liu , Wenhua Tan , Xingqiang Wu , Zhongxing Wu , Gongliang Yu , Renhui Li
DOI:
Received March 15, 2010,Revised June 30, 2010, Accepted , Available online
Volume 23,2011,Pages 102-107
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A water bloom sample collected from Lake Dishui in Shanghai was characterized. The morphological identification showed that
Micorcystis wesenbergii and Micorcystis smithii were the main component of the bloom. Five strains of M. smithii were successfully
isolated. Their 16S rRNA gene sequences based phylogenetic tree showed that the five strains of M. smithii intermixed with strains
of other morphospecies in Microcystis. A fragment of mcy gene encoding for microcystin synthetase was detected in one of the five
M. smithii strains (CHAB 2183), indicating its potential of microcystin production. High performance liquid chromatography analysis
confirmed M. smithii CHAB 2183 to produce microcystin-RR as 1550 g per gram dry weight cells. The present investigation, for the
first time, reported the isolated strains of M. smithii and microcystin production from M. smithii.
