Biodegradation of acrylamide by Enterobacter aerogenes isolated fromwastewater in Thailand
Kanokhathai Buranasilp , Jittima Charoenpanich
DOI:
Received April 26, 2010,Revised July 09, 2010, Accepted , Available online
Volume 23,2011,Pages 396-403
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A widespread use of acrylamide, probably a neurotoxicant and carcinogen, in various industrial processes has led to environmental
contamination. Fortunately, some microorganisms are able to derive energy from acrylamide. In the present work, we reported the
isolation and characterization of a novel acrylamide-degrading bacterium from domestic wastewater in Chonburi, Thailand. The strain
grew well in the presence of acrylamide as 0.5% (W=V), at pH 6.0 to 9.0 and 25°C. Identification based on biochemical characteristics
and 16S rRNA gene sequence identified the strain as Enterobacter aerogenes. Degradation of acrylamide to acrylic acid started in the
late logarithmic growth phase as a biomass-dependent pattern. Specificity of cell-free supernatant towards amides completely degraded
butyramide and urea and 86% of lactamide. Moderate degradation took place in other amides with that by formamide > benzamide >
acetamide > cyanoacetamide > propionamide. No degradation was detected in the reactions of N,N-methylene bisacrylamide, sodium
azide, thioacetamide, and iodoacetamide. These results highlighted the potential of this bacterium in the cleanup of acrylamide/amide
in the environment.
