Arsenic redox transformation by Pseudomonas sp. HN-2 isolated from arsenic-contaminated soil in Hunan, China
Zhennan Zhang
,
Naiyi Yin
,
Xiaolin Cai
,
Zhenzhou Wang
,
Yanshan Cui
DOI:10.1016/j.jes.2015.11.036
Received September 17, 2015,Revised October 12, 2015, Accepted November 04, 2015, Available online April 08, 2016
Volume 28,2016,Pages 165-173
A mesophilic, Gram-negative, arsenite[As(III)]-oxidizing and arsenate[As(V)]-reducing bacterial strain, Pseudomonas sp. HN-2, was isolated from an As-contaminated soil. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the strain was closely related to Pseudomonas stutzeri. Under aerobic conditions, this strain oxidized 92.0% (61.4 μmol/L) of arsenite to arsenate within 3 hr of incubation. Reduction of As(V) to As(III) occurred in anoxic conditions. Pseudomonas sp. HN-2 is among the first soil bacteria shown to be capable of both aerobic As(III) oxidation and anoxic As(V) reduction. The strain, as an efficient As(III) oxidizer and As(V) reducer in Pseudomonas, has the potential to impact arsenic mobility in both anoxic and aerobic environments, and has potential application in As remediation processes.
