Biodegradation of 3,5-dimethyl-2,4-dichlorophenol in saline wastewater by newly isolated Penicillium sp. yz11-22N2
Zhou Yan , Huijun He , Chunping Yang , Guangming Zeng , Le Luo , Panpan Jiao , Huiru Li , Li Lu
DOI:10.1016/j.jes.2017.02.012
Received November 05, 2016,Revised February 16, 2017, Accepted February 17, 2017, Available online February 28, 2017
Volume 29,2017,Pages 211-220
In this study, the performance of 3,5-dimethyl-2,4-dichlorophenol (DCMX) degradation by a screened strain was investigated. 18S rDNA and the neighbor-joining method were used for identification of the isolated strain. The results of phylogenetic analysis and scanning electron micrographs showed that the most probable identity of the screened strain should be Penicillium sp. Growth characteristics of Penicillium sp. and degradation processes of DCMX were examined. Fourier transform infrared spectroscopy of the inoculated DCMX solution was recorded, which supported the capacity of DCMX degradation by the screened Penicillium sp. Under different salinity conditions, the highest growth rate and removal efficiency for DCMX were obtained at pH 6.0. The removal efficiency decreased from 100% to 66% when the DCMX concentration increased from 5 to 60 mg/L, respectively. Using a Box–Behnken design, the maximum DCMX removal efficiency was determined to be 98.4%. With acclimation to salinity, higher removal efficiency could be achieved. The results demonstrate that the screened Penicillium sp. has the capability for degradation of DCMX.
