Analysis of key microbial community during the start-up of anaerobic ammonium oxidation process with paddy soil as inoculated sludge
Xianglong Xu , Guohua Liu , Yuanyuan Wang , Yuankai Zhang , Hao Wang , Lu Qi , Hongchen Wang
DOI:10.1016/j.jes.2017.06.026
Received March 28, 2017,Revised June 05, 2017, Accepted June 21, 2017, Available online July 03, 2017
Volume 30,2018,Pages 317-327
A sequencing batch reactor (SBR)-anaerobic ammonium oxidation (anammox) system was started up with the paddy soil as inoculated sludge. The key microbial community structure in the system along with the enrichment time was investigated by using molecular biology methods (e.g., high-throughput 16S rRNA gene sequencing and quantitative PCR). Meanwhile, the influent and effluent water quality was continuously monitored during the whole start-up stage. The results showed that the microbial diversity decreased as the operation time initially and increased afterwards, and the microbial niches in the system were redistributed. The anammox bacterial community structure in the SBR-anammox system shifted during the enrichment, the most dominant anammox bacteria were Candidatus Jettenia. The maximum biomass of anammox bacteria achieved 1.68 × 109 copies/g dry sludge during the enrichment period, and the highest removal rate of TN achieved around 75%.
