Woody species Rhus chinensis Mill. seedlings tolerance to Pb: Physiological and biochemical response

Xiang Shi , Shufeng Wang , Dongxue Wang , Haijing Sun , Yitai Chen , Jianfeng Liu , Zeping Jiang


Received November 04, 2017,Revised , Accepted July 10, 2018, Available online July 19, 2018

Volume 31,2019,Pages 63-73

Screening potential plant species is a crucial consideration in phytoremediation technology. Our previous study demonstrated that Rhus chinensis Mill. seedlings had potentials for phytoremediation of Pb contaminated soil. However, its bioaccumulation and tolerance characteristics remain unclear. Seedling growth, LMWOAs secreted by roots, Pb subcellular distribution and chemical forms, and mineral elements in R. chinensis tissues were evaluated under different Pb concentrations (0, 25, 50, 100, 200 and 400 mg/L) in culture solution at 14 days after planting. R. chinensis did not show visual symptoms of Pb toxicity under lower Pb treatments; however, Pb significantly declined the growth of seedlings under higher Pb treatments. Higher Pb stress also decreased the concentrations of nitrogen in leaves, but increased the concentrations of P and K in roots. Pb stress also decreased Mn concentrations in leaves. A great quantity of Pb was uptake and mostly retained in R. chinensis roots. Nonetheless, R. chinensis can still concentrate 459.3 and 1102.7 mg/kg Pb in leaves and stems, respectively. Most of Pb in R. chinensis tissues was stored in the cell wall with HAc-, HCl-, and NaCl-extractable form. LMWOAs secreted by R. chinensis roots showed a strong positive correlation with Pb concentrations in all plant tissues and with P in roots. Our results suggested that Pb deposited in the cell wall and integration with phosphate or oxalate might be responsible for the tolerance of R. chinensis under Pb stress in short period.

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